Journal: Cell & Bioscience

Article Title: Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

doi: 10.1186/s13578-025-01401-1

Figure Lengend Snippet: Results of MIR-ASL scanning and CD31 expression detection in the brains of nude mice. A Representative MRI-T2WI film showing the EMS surgical site (white arrow indicates the EMS) and MIR-ASL films showing differences in CBP between the various groups (white dashed lines indicate ROIs under the EMS surgical sites). B Column chart showing the perfusion ratios (EMS sides over non-EMS sides) of each group (n = 6, one-way ANOVA and Šídák's multiple comparisons test). C Representative western blot showing the relative expression of CD31 in the CIBT adjacent to the TM tissue in each group (normalized to β-actin expression). D Densitometric analyses of the relative expression of CD31 (n = 3, one-way ANOVA and Šídák's multiple comparisons test). E Representative confocal images showing the expression of CD31 in the CIBT in each group. Bar = 20 μm. F Column chart showing the counts of CD31 + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). The error bars represent the ± SDs. ASL: arterial spin labelling; CBP: cerebral blood perfusion; CIBT: chronically ischaemic brain tissue; EMS: encephalomyosynangiosis; MRI: magnetic resonance imaging; ROIs: regions of interest; TM: temporal muscle; T2WI: T2-weighted imaging

Article Snippet: The cell suspension was subsequently mixed with 5 μL of Annexin V-FITC staining solution and 10 μL of propidium iodide staining solution at room temperature in a dark room for 15 min. For direct coculture, anti-CD31 (10148-MM13-A, Sino Biological, China) was also used to label the HUVECs.

Techniques: Expressing, Western Blot, Magnetic Resonance Imaging, Imaging

Journal: Cell & Bioscience

Article Title: Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

doi: 10.1186/s13578-025-01401-1

Figure Lengend Snippet: Immunofluorescence and western blotting results showing the expression of relevant cytokines in the CIBT of 2VO + EMS rats. A Representative triple immunofluorescence staining images showing ANGPT2 + , Tie2 + , and CD11b + cells in the CIBT in each group (white arrows indicate TEMs). Bar = 50 µm. B Counts of ANGPT2 + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). C Counts of TEMs (Tie2 + and CD11b + ) in each group (n = 9, one-way ANOVA and Šídák's multiple comparisons test). D Representative western blot showing the relative expression of TRPS1, ANGPT2, Tie2, CD11b, VEGFA, IGF1, and CD31 in the CIBT adjacent to the TM tissue in each group (normalized to β-actin expression). E Densitometric analyses of the relative expression of TRPS1, ANGPT2, Tie2, CD11b, VEGFA, IGF1, and CD31 (n = 3, one-way ANOVA and Šídák's multiple comparisons test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). The error bars represent the ± SDs. CIBT: chronically ischaemic brain tissue; EMS: encephalomyosynangiosis; TEMs: Tie2-expressing monocytes/macrophages; 2VO: 2-vessel occlusion

Article Snippet: The cell suspension was subsequently mixed with 5 μL of Annexin V-FITC staining solution and 10 μL of propidium iodide staining solution at room temperature in a dark room for 15 min. For direct coculture, anti-CD31 (10148-MM13-A, Sino Biological, China) was also used to label the HUVECs.

Techniques: Immunofluorescence, Western Blot, Expressing, Staining

Journal: Cancers

Article Title: Local Magnetic Hyperthermia and Systemic Gemcitabine/Paclitaxel Chemotherapy Triggers Neo-Angiogenesis in Orthotopic Pancreatic Tumors without Involvement of Auto/Paracrine Tumor Cell VEGF Signaling and Hypoxia

doi: 10.3390/cancers16010033

Figure Lengend Snippet: Chemotherapy in combination with magnetic hyperthermia significantly decreased the number of endothelial cells in residual pancreatic tumors. ( a ) Semi-quantitative analysis of the tumor’s CD31 expression levels. ( b ) Representative micrographs showing the number of CD31-positive cells in tumor tissue slices of the various treatment groups. Each bar represents mean ± SEM. Eight images were quantified per treatment group ( n = 2 mice/group for CD31 expression analysis). Positive stains were computed relative to the nuclei number in a ROI stained with hematoxylin. Scale bar: 20 µm. Animal groups: MHsC = magnetic hyperthermia + chemotherapy, MH = magnetic hyperthermia, sC = systemic chemotherapy, M = MNP alone, and N = non-treated. * p < 0.05, ** p < 0.01.

Article Snippet: These were incubated with primary antibodies against human VEGFR2 (1:800, Cell Signaling Technology Inc., Danvers, MA, USA), mice CD31 (PECAM-1, 1:200, Cell Signaling Technology Inc., MA, USA), human/mouse HIF-1α (1:100, Cell Signaling Technology Inc., MA, USA), human Neuropilin-1, human α v β 3 integrin (1:200, Abcam plc, Cambridge, UK), human VEGF (1:100, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), or Ki67 (1:500, Abcam plc, Cambridge, UK), followed by incubation with a biotin-labeled secondary antibody (goat anti-rabbit IgG (H + L), 1:2250, Dianova GmbH, Hamburg, Germany) or with a peroxidase-labeled polymer secondary antibody (DAKO, Glostrup, Denmark).

Techniques: Expressing, Staining

Journal: Cancers

Article Title: Local Magnetic Hyperthermia and Systemic Gemcitabine/Paclitaxel Chemotherapy Triggers Neo-Angiogenesis in Orthotopic Pancreatic Tumors without Involvement of Auto/Paracrine Tumor Cell VEGF Signaling and Hypoxia

doi: 10.3390/cancers16010033

Figure Lengend Snippet: Bimodal therapy leads to a greater number of smaller blood vessels in residual pancreatic tumors than other treatments. ( a ) Histogram showing distribution of tumor blood vessels with regard to their diameters. The bimodal therapy group revealed a higher number of smaller tumor vessels of 0–5 µm diameter (area of analyzed ROI: 9.3 mm²) (MHsC, yellow arrow) as compared to those from the other treatment groups. ( b ) Semi-quantitative evaluation of CD31-positive tumor vessel diameter (0–120 µm). A bin is a bar whose height reflects the number of data points it contains; a bin center is the center value of the grouped data. Each box-plot represents mean ± SEM ( n = 100 vessels/group). Animal groups: MHsC = magnetic hyperthermia + chemotherapy, MH = magnetic hyperthermia, sC = systemic chemotherapy, M = MNP alone, and N = non-treated. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: These were incubated with primary antibodies against human VEGFR2 (1:800, Cell Signaling Technology Inc., Danvers, MA, USA), mice CD31 (PECAM-1, 1:200, Cell Signaling Technology Inc., MA, USA), human/mouse HIF-1α (1:100, Cell Signaling Technology Inc., MA, USA), human Neuropilin-1, human α v β 3 integrin (1:200, Abcam plc, Cambridge, UK), human VEGF (1:100, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), or Ki67 (1:500, Abcam plc, Cambridge, UK), followed by incubation with a biotin-labeled secondary antibody (goat anti-rabbit IgG (H + L), 1:2250, Dianova GmbH, Hamburg, Germany) or with a peroxidase-labeled polymer secondary antibody (DAKO, Glostrup, Denmark).

Techniques:

Journal: Current protocols in chemical biology

Article Title: Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging

doi: 10.1002/cpch.14

Figure Lengend Snippet: Antibodies tested in the CycIF protocol

Article Snippet: *** p-ERK1/2 T202/Y204 (CST #4344, Lot #12): 1:200 , *** p-Rb S807/S811 (CST #8957, Lot #1): 1:400 , *** p21 Waf1/Cip1 (CST #8587, Lot #3): 1:200.

Techniques:

Journal: Current protocols in chemical biology

Article Title: Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging

doi: 10.1002/cpch.14

Figure Lengend Snippet: An example of 6-round CycIF experiment using both indirect and direct IF

Article Snippet: *** p-ERK1/2 T202/Y204 (CST #4344, Lot #12): 1:200 , *** p-Rb S807/S811 (CST #8957, Lot #1): 1:400 , *** p21 Waf1/Cip1 (CST #8587, Lot #3): 1:200.

Techniques: